Journal: Journal of Bacteriology

Article Title: The Stigmatella aurantiaca Homolog of Myxococcus xanthus High-Mobility-Group A-Type Transcription Factor CarD: Insights into the Functional Modules of CarD and Their Distribution in Bacteria

doi: 10.1128/jb.185.12.3527-3537.2003

Figure Lengend Snippet: FIG. 5. Southern hybridization analysis for the presence of carD- like genes in other myxobacteria and in bdellovibrios. (A) XhoI-di- gested genomic DNAs from M. xanthus (Mx), M. coralloides (Mc), C. fuscus (Cf), S. aurantiaca (Sa), N. exedens (Ne), and P. cellulosum (Pc) probed with a DNA fragment corresponding to nucleotides 354 to 948 of the carD gene. (B) Genomic DNAs from M. xanthus (Mx) and Bdellovibrio sp. strain CP41 (Bd) digested with the indicated restriction enzyme and probed with the same DNA fragment as for panel A.

Article Snippet: Myxococcus (Corallococcus) coralloides, Cystobacter fuscus, Nannocystis exedens, and Polyangium cellulosum were obtained from the culture collection of Deutsche Sammlung von Mikroorganism und Zellkulturen GmbH, Braunschweig, Germany, and grown in the following media: C. fuscus and M. coralloides, 0.1% each raffinose, sucrose, and galactose, 0.25% Casitone, 0.5% starch, 0.05% MgSO4 7H2O, and 0.025% K2HPO4 (pH 7.4); N. exedens, 0.5% dry baker’s yeast, 0.14% CaCl2 2H2O, and 0.5 g of vitamin B12 per ml (pH 7.2); and P. cellulosum, 0.1% KNO3, 0.1% K2HPO4, 0.03% MgSO4 7H2O, 0.013% CaCl2 2H2O, 0.003% FeCl3 6H2O, and small strips of filter paper (pH 7.2).

Techniques: Hybridization